Urea is a product of amino acid that is produce when amino acid is broken down by the liver. Urea Normal Range. In Europe, the whole urea molecule is measured. Get a printable copy (PDF file) of the complete article (594K), or click on a page image below to browse page by page. Serum urea reacts with urease and water to liberate ammonia and carbon dioxide. The normal blood (serum) urea = 10-50 mg/100ml of blood. The liberated ammonia changes the pH of the reaction medium, which is monitored by the chromogen phenol red. Normal value of blood urea. A single step working reagent strip has been developed and the reaction is completed within 50 seconds at room temperature. It is eliminated from the body almost exclusively by the kidneys in urine, and measurement of its concentration, first in urine and later in blood, has had clinical application in the assessment of kidney (renal) function for well over 150 years. In this method for serum and urinary urea determination, the same reagent is used without predilution of urine samples. Now in the givenpatient, the urea clearance is 25 ml/minute means only 25 ml of plasma isgetting completely cleared of urea per minute, this is very low in comparisonto the normal clearance values, that means kidney is failing to excrete urea inurine , hence it is getting accumulatedin blood to cause high urea level, as isapparent from the high level of 54 mg/dl in this patient. Full text Full text is available as a scanned copy of the original print version. Fasting blood glucose, blood urea, serum creatinine were measured by enzymatic method. After urea is formed in the liver, it passes into the blood and then excreted in the urine. In the United States only the nitrogen component of urea is measured (the blood urea nitrogen, i.e., BUN), which is roughly one-half of blood Urea is the principal nitrogenous waste product of metabolism and is generated from protein breakdown. Normal human blood should contain between 1.8 – 7.1 mmol/L . The concentration of urea in blood depends upon the relationship between urea production and urea excretion. BLOOD ESTIMATION OF UREA Almost half of non proteinous nitrogen substance found in the blood is the constituent of urea. Estimation of urea in blood serum Principle: Urea is synthesized in the liver from the ammonia produced mostly by the catabolism of amino acids. Particle-enhanced Turbidimetric inhibition immunoassay (PETINIA) was used to determine albumin in urine.
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